Figure 4

Staining of CD127 and CD69 on CD8 cells and detection of CD4 + cells from NP immunized mice with or without TAK-779 treatment. Two weeks after TAK-779 cessation, splenocytes were prepared from different mice groups and analyzed by flow cytometry after staining with anti-Mouse CD8a-APC, anti-Mouse CD127-FITC, anti-Mouse CD69-PE, anti-Mouse CD3e-PE-cy™ 7, anti-Mouse CD4-FITC or isotype control antibodies. Percentage of CD8 + CD127 + (A and C) and CD8 + CD69 + (B and D) cells in mice immunized once (A and B) or twice (C and D) with NP with or without TAK-779 treatment were analyzed by flow cytometry and compared with mice from the control group. Percentage of CD3 + CD4 + cells immunized once or twice with NP is illustrated in (E) and (F), respectively. NP: 1 NP immunization; NP2: 2 NP immunizations; TN: 1 NP immunization with TAK-779 treatment; TN2: 2 NP immunizations with TAK-779 treatment. Results are expressed as the mean ± SD. * p ≤ 0.05; *** p ≤ 0.001. The data are representative of 2 independent experiments with at least 4 mice per group. Mice were analyzed individually.