Proliferative ability of CD8 + T cells in NP-immunized mice with or without TAK-779 treatment compared with control group. Two weeks after TAK-779 cessation, splenocytes were prepared from individual spleens collected from naïve mice, mice treated with TAK-779 (TAK), mice immunized once (NP) or twice with NP (NP2) and mice immunized once (TN) or twice (TN2) with NP and treated with TAK-779. Proliferation was measured as mean fluorescence intensity (mfi) of CFSE by flow cytometry upon NP stimulation. (A) One NP immunization; (B) 2 NP immunizations. Results are expressed as the mean ± SD. The data are representative of 2 independent experiments with at least 4 mice per group. Mice were analyzed individually.