Figure 8

Protection efficacies of mice immunized with NP with or without TAK-779 treatment against lethal H1N1 influenza A virus challenge Mice were immunized once or twice with NP and treated with or without TAK-779 as scheduled. Two weeks after TAK-779 cessation, mice were challenged with 10 LD50 of H1N1 (A/FM/1/47). The survival rates (A) were compared with infected control mice (naïve and TAK groups). Body-weight changes (B) were monitored daily, and mice were euthanized when body weight loss exceeded 25% of original body weight. (C) Viral load quantification in the lungs of control mice and mice immunized twice with NP and treated with or without TAK-779. Mice were immunized twice with NP and treated with or without TAK-779 as scheduled. Two weeks after TAK-779 cessation, mice were challenged with 10 LD50 of H1N1 (A/FM/1/47). At 3 d post-viral challenge, the viral loads were measured in the lungs of different groups of mice by TCID50 assay. Each symbol represents an individual mouse, and the lines represent the geometric mean ± SD. Five animals per group per time point were assayed. *** p ≤ 0.001.