Figure 7

Effects of WISP1 on poly(I:C)-induced TNF-α production in macrophages. (A) Peritoneal macrophage isolated from C57BL/6J mice were treated with WISP1 (10 µg/mL) in the presence or absence of various concentrations (0–10 µg/mL) of poly(I:C) in serum-free medium for 16 h. TNF-α levels in the supernatant were measured by ELISA. (B) Peritoneal macrophage isolated from C57BL/6J mice were treated with WISP1(10 µg/mL) in the presence or absence of poly(I:C) (5 µg/mL) in serum-free medium for up to 24 h. TNF-α levels in the supernatant were measured by ELISA at 0 h, 4 h, 8 h, 12 h, 16 h, 20 h and 24 h after stimulation. Data are presented as mean ± SEM (n = 5) and compared by two-way ANOVA. For comparing the poly(I:C) + WISP1 group with the poly(I:C) alone group *P < 0.05, ***P < 0.001.