Figure 9

Effects of WISP1 and poly(I:C) on ERK phosphorylation in macrophages (A) Peritoneal macrophages isolated from C57BL/6J mice were treated with WISP1 (10 µg/mL) or poly(I:C) in serum free medium for 15, 30 and 60 min. ERK phosphorylation was detected by Western blot. Effects of an ERK inhibitor, U0126, on WISP1 and poly(I:C)-induced TNF-α release in macrophages. (B) Peritoneal macrophage isolated from C57BL/6J mice were treated with WISP1 (10 µg/mL) and poly(I:C) (5 µg/mL) in the presence or absence of various concentrations (0–1.0 µmol/L) of U0126 in serum free medium for 16 h. TNF-α levels in the supernatant were measured by ELISA. Data are presented as means ± SEM (n = 5) and compared by two-way ANOVA. For comparing the poly(I:C) + WISP1 group pretreated with U0126 0.5 µmol/L with poly(I:C) + WISP1 untreated with U0126 group ***P < 0.001.