Figure 1

Generation of a HLA-universal iPSC line. iPSCs cultured in monolayer on Laminin-521-coated plates were transduced with a lentiviral vector (pLVTHm) encoding the sequences for a nonspecific shRNA (shNS) or a shRNA targeting β2m transcripts (shβ2m). (A) Levels of β2m transcripts measured by real time PCR before and 5 d after cell transduction (Td). GAPDH was used as housekeeping gene to normalize cDNA levels. (B) Expression levels of HLA class I measured by flow cytometric analysis prior transduction and at passages (P) 2, 5, 10 and 15. Graphs show mean ± standard deviation (SD) (n = 4, ***p ≤ 0.001).