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Figure 2 | Molecular Medicine

Figure 2

From: HMGB1 Mediates Anemia of Inflammation in Murine Sepsis Survivors

Figure 2

Sepsis survivors have defective terminal erythroid differentiation. Spleen and bone marrow were obtained from sepsis survivors 10 d after mice were subjected to CLP or sham surgery. CLP survivors developed splenomegaly (A) and an expansion of the red pulp (B), suggesting stress erythropoiesis. Dash borders denote the separation between the red pulp (rp) and the white pulp (wp). Numerical apertures for the different magnifications: 10× = 0.45; 20× = 0.80; 40× = 0.75; 63× oil = 1.4. Scale bar: 125 µm (10×), 62 µm (20×), 31 µm (40×) and 20 µm (63×). Analysis of the erythroid compartment by flow cytometry shows an expansion of immature erythroid cells in the spleen of CLP animals (C, D). Bone marrow total cellular content was not different between groups, either before or after depletion (E). Flow analysis shows an increase in orthochromatic erythroblasts (F, G). Cell counts of total and post-depletion bone marrow represents the number of cells from femur and tibia obtained from both hind limbs. Data shown are mean ± SD (n = 3−4 mice/group).

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