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Figure 3 | Molecular Medicine

Figure 3

From: HMGB1 Mediates Anemia of Inflammation in Murine Sepsis Survivors

Figure 3

HMGB1 induces anemia and impairs late erythropoiesis. BALB/c mice were injected with rHMGB1 (500 µg/mouse/d, intraperitoneally) or vehicle for 7 d; blood, spleen and bone marrow were collected 24 h after the last injection. (A) HMGB1-induced anemia with an elevated reticulocyte count. (B) Bone marrow total cellular content was not different between groups, but the erythroid compartment was significantly reduced. (C, D) Analysis of the erythroid compartment by flow cytometry shows an expansion of terminal erythroid precursors. (E) Administration of HMGB1 also induced splenomegaly (scale bar: 1 cm). (F) Analysis of the splenic cell compartment showed that the total cell count, as well as the erythroid compartment (post-CD45 depletion), were significantly increased. (G, H) Flow cytometry analyses indicated an increase in the different erythroid populations. Data are expressed as box and whisker plots (A) or mean ± SD (B, D, F, H) (n = 3−4 mice/group). *p < 0.05, **p < 0.01, ***p < 0.001 versus vehicle.

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