Figure 1

Annexin A5 treatment enhances survival in LPS-induced and cecal content injection-induced sepsis model. (A) endotoxemia model: C57BL/6 mice were intraperitoneally injected with lethal doses of LPS, 50 mg/kg. Concurrently, each mouse was injected intravenously with 500 µg annexin A5 (in 100 µL PBS) and monitored for 50 h. * — indicates significant differences (P < 0.05) from data obtained during the LPS-only challenge. (B) cecal content injection model: C57BL/6 mice were intraperitoneally injected with 20 mg of cecal contents. Each mouse was intravenously injected with 500 µg (in 100 µL PBS) annexin A5 protein concurrently with CCI challenge and monitored for 50 h. * — Indicates significant differences (P < 0.05) from data obtained during the CCI challenge. In both (A) and (B), the line graph illustrates survival of C57BL/6 mice in different treatment groups over time. We monitored survived mice in each group for another three weeks, no unexpected death was observed. Each group consisted of 6 mice and the experiments were performed twice. Annexin A5 is abbreviated as “Ann V” and control group as “con.”