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Figure 3 | Molecular Medicine

Figure 3

From: Annexin A5 Increases Survival in Murine Sepsis Model by Inhibiting HMGB1-Mediated Proinflammation and Coagulation

Figure 3

Annexin A5 reduces proinflammatory cytokines as well as the extent of organ dysfunction and tissue damage in LPS-induced sepsis model. C57BL/6 mice were challenged with 50 mg/kg LPS, followed by simultaneous intravenous injection of 500 µg (in 100 µl PBS) annexin A5 protein. (A) proinflammatory cytokine level: whole blood was collected by cardiac puncture and serum was extracted 1, 3, 12 and 18 h after the treatment. Serum concentrations of proinflammatory cytokines IL6 and TNFα were determined using ELISA. Cytokine concentrations are illustrated as line plots. Data represent the mean ± S.E.M. of three independent experiments. * — Indicates significant differences (P < 0.05) at each time point between the LPS-only and annexin A5 + LPS treatments. (B) extent of organ dysfunction: whole blood was collected by cardiac puncture and serum was extracted 18 h after the treatment. Concentrations of AST, ALT and BUN in serum samples were measured and the results are represented by bar graphs. Data represent the mean ± S.E.M. of three independent experiments. “No treatment” group at 0 h was used for control values. * — Indicates significant differences (P < 0.05) at each time point between the LPS-only and annexin A5 + LPS treatments. (C) degree of tissue damage: 18 h after the treatment, lungs were excised, perfused, and fixed with formalin. Lung sections were stained with hematoxylin and eosin. Representative images of lung sections from each group were captured with a digital camera (Nikon DS-Ri) coupled to a Nikon Eclipse Ni microscope under 20× magnification. Scale bar, 100 µm.

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