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Figure 5 | Molecular Medicine

Figure 5

From: Annexin A5 Increases Survival in Murine Sepsis Model by Inhibiting HMGB1-Mediated Proinflammation and Coagulation

Figure 5

Annexin A5 reduces LPS- and HMGB1-mediated pro-coagulation in septic conditions. (A) LPS-challenged group: C57BL/6 mice were intraperitoneally injected with 50 mg/kg LPS. Concurrently, each mouse was intravenously injected with 500 µg (in 100 µL PBS) annexin A5 protein. Twelve hours after the treatment, the distal 1 cm-long portion of the tail tip was amputated. (B) delayed treatment group: C57BL/6 mice were intraperitoneally injected with 50 mg/kg. Three hours after the LPS challenge, each mouse was intravenously injected with 500 µg (in 100 µL PBS) annexin A5. Twelve hours after treatment, the distal 1 cm-long portion of the tail tip was amputated. (C) HMGB1-challenged group: C57BL/6 mice were intravenously injected with the mixture of 300 µg HMGB1 and 500 µg annexin A5 in 100 uL PBS. Three hours after the treatment, the distal 1 cm-long portion of the tail tip was amputated. (A-C) Volume of blood flowed out of tail (in µL) and the time of cessation of blood flow (in seconds) were monitored and illustrated on the bar graph. Each group consisted of 3 mice and the experiments were performed twice. * — Indicates significant differences (P < 0.05) from data obtained during the LPS- or HMGB1-only challenge.

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