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Figure 2 | Molecular Medicine

Figure 2

From: Interleukin-26 Production in Human Primary Bronchial Epithelial Cells in Response to Viral Stimulation: Modulation by Th17 cytokines

Figure 2

The adaptor protein TRIF is involved in poly-IC-induced release of IL-26. Primary bronchial epithelial cells were preincubated (5 h) with TRIF inhibitor and vehicle (25 pmol/L). Extracellular concentrations of IL-26 in cell-free conditioned media as well as intracellular levels were measured using ELISA and western blot respectively and mRNA levels using real time. (A) Extracellular concentrations of IL-26 in cell-free conditioned in response to poly-IC (0.05 pg/mL) in the presence of the TRIF inhibitor (n = 7). (B) Level of IL26 mRNA in response to poly-IC (lpg/mL) in the presence of the TRIF inhibitor (n = 8). (C) Intracellular IL-26 protein (representative western blot) and (D) the average protein expression (fold difference) in response to poly-IC (1 pg/mL) in the presence of the TRIF inhibitor (n = 4).

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Molecular Medicine

ISSN: 1528-3658