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Figure 3 | Molecular Medicine

Figure 3

From: Interleukin-26 Production in Human Primary Bronchial Epithelial Cells in Response to Viral Stimulation: Modulation by Th17 cytokines

Figure 3

Stimulation with poly-IC induces phosphorylation of MAP kinases and NF-κB and is inhibited by TRIF. Cells were preincubated with the TRIF inhibitor (ihh-TRIF) or vehicle (V-TRIF) (25 µmol/L for 5 h) and/or stimulated with poly-IC (0.05 µg/mL or 0.5 µg/mL) for another 1.5 h. Adherent cells were then lysed and the lysate used to measure phosphorylated levels of p38, JNK1-3, ERK1/2 and NF-κB by phosphorTracer ELISA. Panels (n = 8) show relative fluorescence units (RFU) of (A) Phosphorylated (p) p38, (B) pJNK1-3, (C) pERK1–3, (D) pNF-κB and (E) comparative levels for the different molecules. The p values indicated are according to Student paired t test and a p < 0.05 is considered significant.

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Molecular Medicine

ISSN: 1528-3658