Figure 5

Th17 cytokines modulate poly-IC induced release of IL-26. Primary bronchial epithelial cells were stimulated with poly-IC (0.05 pg/mL) in the presence or absence of rhIL-17A (100 ng/mL) or rhIL-22 (100 ng/mL) or rhIL-17A (100 ng/mL) plus rhIL-22 (100 ng/mL) (24 h). Extracellular concentrations of IL-26 in cell-free conditioned media as well as intra-cellular levels were measured using ELISA and western blot respectively and mRNA level by real time PCR. (A) Extracellular concentrations of IL-26 in cell-free conditioned media in response to rhIL-17A and/or rhIL-22 (n = 14). (B) IL26 mRNA level in response to rhIL-17A (n = 9). (C) Extracellular concentrations of IL-26 in cell-free conditioned media in response to poly-IC plus rhIL-17A (n = 6). (D) Extracellular concentrations of IL-26 in cell-free conditioned media in response to poly-IC plus IL-22 (n = 6). (E) Extracellular concentrations of IL-26 in cell-free conditioned media in response to poly-IC plus rhIL-17A plus rhIL-22. (n = 6). (F) Intracellular IL-26 protein (representative western blot) and (G) the average protein level (fold difference) in response to IL-17A during 24 h (n = 10). The p values indicated are cording to the Student paired t test. p < 0.05 is considered significant