Figure 2

Gene expression of Mmp12/MMP12 in obese mouse/human adipose tissue. Total RNA was extracted from GWAT and SWAT from mice (A and B) and humans (C), respectively. (A) Mmp12 expression in GWAT of WT mice fed HFD or CD from the microarray experiment was analyzed by RT-PCR with mice on CD being set to 1; n = 12 mice of each condition; (B) Mmp12 expression in GWAT and SWAT of WT mice fed HFD or CD was analyzed by RT-PCR in a separate experiment with mice on CD being set to 1; n = 15 mice of each condition; (C) MMP12 expression in OWAT and SWAT from obese and lean subjects, OWAT of lean human was set to 1; n = 12 of each group. Results are expressed as mean ± SEM. * p < 0.05; ** p < 0.01; *** p < 0.001 and **** p < 0.0001 compared with CD (A, B) or lean (C), respectively.