Figure 3

BD750 inhibits proinflammatory cytokine secretion and STAT5 phosphorylation in DCs. BMDCs were stimulated with cytokines for expansion in the presence or absence of BD750 for 12 h and then stimulated with LPS for 24 h. The supernatants of cultured cells were harvested and the levels of IL-6, IL-10, IL-12, TNF-α, IL-1β and IL-23 in the supernatants were determined by ELISA (A-F). In addition, the relative levels of STAT5 and AKT expression and phosphorylation in the different groups of DCs were determined by Western blot assays (G-H). The relative levels of STAT5 and AKT phosphorylation to total STAT5 and AKT were determined. The relative density for bands of p-STAT5 or p-AKT activity was normalized to the total STAT5 or AKT, respectively. Data are representative images or expressed as the mean ± SEM of three independent experiments. *P < 0.05, **P < 0.01 versus the mDC group.