Figure 5

C1213 and CNI-1493 attenuate in vivo Aβ 1-42 toxicity in C. elegans. (A) Western blot of the cytosolic fraction of amyloid precursor protein overexpressing SH-SY5Y shows a band consistent with cytosolic Aβ that is not present in wild-type cells. (B) Western blot of the CL2006 C. elegans model of muscular Aβ 1-42 overexpression and paralysis shows Aβ reactivity in worm lysates. (C) CL2006 Aβ 1-42 overexpressing worms were stained with Thioflavin S and visualized using confocal microscopy. (D) CNI-1493 and C1213 attenuate Aβ-induced paralysis in the CL4176 C. elegans model of muscular Aβ 1-42 overexpression and paralysis. ** P < 0.01 Veh vs. Compound; *** P < 0.001 Veh vs. Compound.