Figure 5
From: α-1 Antitrypsin Inhibits RANKL-induced Osteoclast Formation and Functions
AAT inhibits RANKL-induced CD9 and DC-STAMP expression. Osteoclast cells were generated by treating the bMM cells with M-CSF (d 0–4) and RANKL (d 4–6 or 7). Cells were treated with AAT (2 mg/mL) at the indicated time frame. (A) Cell surface CD9 levels (mean fluorescence intensity) detected by flow cytometry at d 6. b Cell surface DC-STAMP detected by flow cytometry at d 6. (C) Cell surface DC-STAMP detected at d 4. (D) Intracellular DC-STAMP was detected by flow cytometry at d 6 after permeabilizing the cells. (E) DC-STAMP mRNA was detected by qPCR. (F) DC-STAMP mRNA was detected by qPCR at d 7. Values are means ± SEM of at least triplicate samples. Data were analyzed using one-way analysis of variance followed by Dunnett’s multiple comparison test. **P <0.001, ***P <0.0001, Student t test was used to compare two samples. #* = P <0.05 using Student t test. %* = P <0.05 using one-tail Student t test.