Figure 3

LPS induces changes in junction protein expression in zebrafish. (A) A schematic representation of the various cellular junction proteins. LPS reduces the transcript abundance of (B) occludin a, (C) occludin b, (D) claudin 5a and (E) claudin 5b, (F) increases the transcript abundance of claudin 2 and (G) has no effect on the transcript abundance of VE cadherin. LPS treatment also (H, I) decreased the transcript and protein levels of ZO-1 and (J) caused disorganized patterning and increased fragmentation of ZO-1 protein. For experiments on transcript levels, an average of 3–5 trials was used, each with a pool of 20 larvae/treatment. For the immunohistochemistry experiments, an average of 3 trials with 4–6 larvae/treatment were used to obtain quantitative data.