Figure 6
From: Development of a Zebrafish Sepsis Model for High-Throughput Drug Discovery
LPS induces blood flow abnormalities in endotoxemic fish. (A, B) LPS exposure in 3 DPF zebrafish larvae leads to marked changes in the microcirculation, characterized by reduced blood flow through the central arteries of the head (as observed through the use of transgenic zebrafish lines and Hb staining; an average of 3 trials with 7 larvae/treatment), microconstriction of the DA (n = 5–8/treatment) and (C, D) reduced circulation through the DA and PCV. (E, F) LPS-treated larvae also show an increased aggregation of thrombocytes in the CCV and major blood vessels, namely DA and PCV (an average of 3 trials with 5–7 larvae/treatment) CtA: central arteries; DLAV: dorsal longitudinal anastomotic vessel; ISA: intersegmental artery; ISV: intersegmental vein; PCV: posterior cardinal vein; DA: dorsal aorta; M: muscle; NT: neural tube; N: notochord; CCV: common cardinal vein.