Skip to main content

Table 1 Primer pairs used in this study

From: Development of a Zebrafish Sepsis Model for High-Throughput Drug Discovery

Gene ID

Primer sequences (5′-3′)

Temp (oC)

Amplicon size (bp)

VE Cadherin

Fwd: TGACGAGGAGGGCGGAGGAG Rev: AGGTCCCACACTGGGCCGAA

58

100

Occludin a

Fwd: TACCATTACTGCGTGGTGGA Rev: TCACTCTGCGCCATAAGATG

58

154

Occludin b

Fwd: TATGTTTGCCTGCGTAGCTG Rev: CGTATCCGTAGCCTCCCATA

58

182

Claudin 5a

Fwd: GGTCATCTCCTCGGTCTTGA Rev: GCACCTGCGGGTTATAGAAG

58

206

Claudin 5b

Fwd: CTCAATGCACCAATTGCATC Rev: TTTTTGGCGTAGGGAACTTG

58

166

Claudin 2

Fwd: CAACACCCTCCTGGGACTAA Rev: GCCAACTCCAGCTACTTTGG

58

163

ZO-1

Fwd: CACGAGACAAACTGGCAAGA Rev: TCCAGCACTGCATGCTTATC

58

178

IL-1

Fwd: TGGACTTCGCAGCACAAAATG Rev: GTTCACTTCACGCTCTTGGATG

58

150

IL-6

Fwd: AGACCGCTGCCTGTCTAAAA Rev: TTTGATGTCGTTCACCAGGA

58

136

TNF

Fwd: GCTGGATCTTCAAAGTCGGGTGTA Rev: TGTGAGTCTCAGCACACTTCCATC

58

138

IL-811

Fwd: GTCGCTGCATTGAAACAGAA Rev: CTTAACCCATGGAGCAGAGG

58

158

IL-812

Fwd: CTACCGAGACGTGGGTGATT Rev: GCTCGGTGAATGGTCATTTT

58

140

IL-10

Fwd: ATTTGTGGAGGGCTTTCCTT Rev: AATGGGAGTTGCAAAACTGG

60

127

HMGB1

Fwd: AGAGGAGGAGGATGACGAGTAG Rev: GGACGAACACAGATGACACTTC

60

232

TLR4a

Fwd: TGCAAAGGCTTGTTCTTGTG Rev: TGAAGGTGGTCATGAATGGA

60

138

TLR4b

Fwd: CTGTGTTGCGAGAGATTGGA Rev: AGGCAGCTAGCAGATGAAGC

60

120

MyD88

Fwd: TAAACGGCTAATCCCTGTCG Rev: TCGAGTCCAGAACCAGACCT

60

118

EF1α

Fwd: GATGCACCACGAGTCTCTGA Rev: TGATGACCTGAGCGTTGAAG

58

158