Adenosine Generated in the Bone Marrow Niche Through a CD38-Mediated Pathway Correlates With Progression of Human Myeloma

Table 3 Phenotype of myeloma cells and cell lines, osteoclasts, osteoblasts and stromal cells.

Enzyme	MM patients^a				Controls^b			Cell lines
Enzyme	MM	OB	OC	BMSC	OB	OC	SC	LP-1	HS-5	SAOS-2
CD38	+	−	−	−	−	−	+/−	+	−	−
CD203a		+	+	+	+/−	+/−	+/−	−	+	+
CD73		+	+	+	+	+	+	−	+/−	+/−
CD39	+	−	+/−	−	−	−	−	−	−	−
CD26	−	nd	+	nd	+/−	+	−	−	−	−
TRACP^c	nd	nd	+	nd	nd	+	nd	nd	nd	nd

Cells from BM aspirates were stained with specific mAbs followed by FITC-labeled anti-mouse Ig. The same was done for the multiple myeloma LP-1, stromal HS-5 and osteoblasts SAOS-2 cell lines. Phenotype was obtained by staining cells with specific mAbs followed by FITC-labeled anti-mouse Ig.
a. data from MM bone biopsies
b. data from normal bone biopsies
c. immunohistochemistry

ISSN: 1528-3658