Figure 5From: Induction of Steatohepatitis (NASH) with Insulin Resistance in Wild-type B6 Mice by a Western-type Diet Containing Soybean Oil and CholesterolDietary cholesterol in soybean oil diet impaired hepatic insulin signaling. Mice received diets as described in Figure 1. Protein levels of (A) insulin receptor substrate 1 (IRS-1) and (B) IRS-2 were determined in liver lysates by Western blot analysis with specific antibodies. Density units were normalized to Fast Green staining, which was verified on the same Western blot membrane as a loading control. (C) Hepatic glycogen content was enzymatically quantified in homogenates (see Materials and Methods section). Values represent means ± standard error of the mean of 5 mice per group. Statistics: One-way analysis of variance with Dunnett’s post hoc test or, where appropriate, Tukey’s post hoc test for multiple comparisons, *p <0.05.Back to article page