Figure 1

Sterile systemic inflammatory responses induced by skin burn injury in mice. Female BALB/c mice (n = 6 mice/group) were burned with electrocautery at 400–430±C for 3 s and their survival was recorded. The skin and lung tissues of burn-injured mice and normal mice were collected at 24 h post burn for histological analysis. (A) Skin pathological changes (hematoxylin and eosin [H&E] staining, magnification x 40) and survival curves of mice. (a′) and (a″) Higher magnification (x 400) of fibroblasts, denoted by arrows, and neutrophils, denoted by arrowheads, in muscular fascia. Scale bars = 200 µm. (B) Lung pathological changes (H&E staining, magnification × 40) and scores. Scale bars = 200 µm. (C) Ratios of Ly6G⁺ cells in WBCs and Ly6G^high in Ly6G⁺ cells. Ly6G⁺ cells represent the neutrophils. Data are represented as mean ^± standard error of the mean (SEM) (n = 3–5 mice/group). (D) The mtDNA copies in plasma of sham or burn-injured mice were detected by qPCR. Data are represented as mean ± SEM (n = 3 mice/group).