Figure 3
Effect of AAAG ODN on the TLR9-IRF5 pathway in burn-injured skin of the mice. The skin tissues collected from sham and burn-injured mice (3/group) at 0.5 h post burn injury were detected for (A) mRNA and (B) protein levels of TLR9, MyD88, NF-κB and IRF5 by qPCR and Western blot, and for (C) IRF5 expression by immunohistochemistry (IHC). Arrows, black arrowheads and blue arrowheads denote keratinocytes in the epidermis, keratinocytes in hair follicles and fibroblasts in dermis, respectively. Brown represents expression of IRF5 protein. The bars in column charts represent SEM for means; *P < 0.05, **P < 0.01, ***P < 0.001. In the IHC images, scale bars correspond to 100 µm and magnification is × 200 (upper panel) and × 400 (lower panel).