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Figure 8 | Molecular Medicine

Figure 8

From: Myosin Light Chain Kinase Knockout Improves Gut Barrier Function and Confers a Survival Advantage in Polymicrobial Sepsis

Figure 8

Effect of genetic deletion of MLCK on systemic bacterial burden and cytokines 24 h after CLP. (A) The amount of bacteria in the blood was similar between MLCK−/− and WT mice following CLP (n = 10/group, p > 0.99). (B) IL-10 levels in the blood were significantly lower in MLCK−/− mice (n = 7–8/group, p = 0.001). (C) No differences in 7 d survival were identified when MLCK−/− mice were given exogenous IL-10 or vehicle (n = 10/group, p = 0.68). (D) The survival benefit conferred after CLP in MLCK−/− disappeared when anti-IL-10 antibody was given to both MLCK−/− and WT mice (n = 10/group, p = 0.05). (E) IL-6 levels were also lower in MLCK−/− mice (n = 9–11/group, p = 0.03). No differences were detected in systemic (F) TNF (n = 8–9/group, p = 0.14), (G) IL-13 (n = 12/group, p = 0.83) and (H) IFN-Y (n = 12/group, p = 0.08).

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