CLL subset 4 IGs do not bind i or I carbohydrate antigens. (A) Glycan sequences of the lipid-linked oligosaccharides pLNH, pLNnH, LNnO and I-octa that were among 76 additional (i- and Fc10.2- antigen-negative probes) in the microarray. Abbreviations: Gal, galactose; GlcNAc, N-acetylglucosamine; Glc, glucose; DH, L-1,2-dihexadecyl-sn-glycero-3-phosphoethanolamine; Cer, ceramide. (B), (C) Microarray analysis of CLL mAbs 141, 282, 183, 240, and 342, in either (B) native bivalent or (C) precomplexed condition. The human anti-i IgM PIA ELL, human anti-I Ma, and the murine IgM Fc10.2 were included as positive controls. The results are the means of fluorescence intensities of duplicate spots, printed at 5 fmol, with error bars representing half the difference between the two values.