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Figure 4 | Molecular Medicine

Figure 4

From: Binding of CLL Subset 4 B Cell Receptor Immunoglobulins to Viable Human Memory B Lymphocytes Requires a Distinctive IGKV Somatic Mutation

Figure 4

Binding of subset 4 IGs to permeabilized and fixed HEp-2 cells and to DNA. (A) Reactivity of subset 4 IGs with permeabilized and fixed HEp-2 cells. Binding of Abs was detected using fluorochrome-labeled goat anti-human IgG pAbs. (B) Binding of native CLL IGs to DNA determined by ELISA. IGHV4-34+ subset 4 mAbs (nos. 183, 342 and 240), IGHV4-34+ non-subset 4 mAbs (nos. 141 and DO8) and subset 2 (IGHV3-21/IGLV3-21) mAb (no. 282) were tested for reactivity with dsDNA and ssDNA by enzyme immunoassay. Antibodies were used at a concentration of 25 ug/mL. Human sera containing antibodies reactive with dsDNA or with ssDNA were used as positive controls. Human serum without antibodies against dsDNA or ssDNA was used as negative control. Data are average ± SD of at least three independent experiments. Following the calculation and result interpretation information provided by the manufacturer, values ≥ 92.6 WHO units/mL for dsDNA binding (dashed blue line) and ≥ 68.6 U/mL for ssDNA binding (dashed red line) were registered as positive.

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