Figure 1
From: miR-210 Protects Renal Cell Against Hypoxia-induced Apoptosis by Targeting HIF-1 Alpha
Systemic hypoxia and local kidney hypoxia induced HIF-1α activation in a rat model. Six-to-eight-week-old male Sprague-Dawley rats were randomly divided into normoxia (n = 8), systemic hypoxia (7500 m, 7 h; n = 8), sham (n = 8) and local kidney hypoxia (ischemia, bilateral, 60 min; n = 8) groups. (A) Changes in HIF-1α pathway genes (HIF-1α, GLUT-1, BNIP3 and NIX) and HIF-2α mRNA expression in vital organs (brain, lung, heart, liver and kidney) of normoxia and systemic hypoxia groups. (B) Changes in HIF-1α, HIF-2α, GLUT-1, BNIP3 and NIX mRNA expression in kidney of sham and local kidney hypoxia groups. All mRNA levels were assessed by real-time polymerase chain reaction and normalized to β-actin. Data are shown as mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001.