Figure 3

miR-210 increased significantly in rat kidney after systemic and local kidney hypoxia, especially in tubular cells. miR-210, another target of HIF-1α, was measured in normoxia (n = 8), systemic hypoxia (7500 m, 7 h; n = 8), sham (n = 8) and local kidney hypoxia (ischemia, bilateral, 60 min; n = 8) groups. (A) miR-210 expression in the vital organs (brain, lung, heart, liver and kidney) was measured after systemic hypoxia. (B) miR-210 expression in (B) kidney and (C) circulation were measured in the four groups. miR-210 expression in isolated primary tubular cells and glomerular cells from renal cortex after (D) systemic hypoxia and (E) local hypoxia. miR-210 expression in isolated primary tubular cells and glomerular cells from renal cortex after systemic hypoxia. miR-210 levels were measured by real-time PCR. To measure circulating miR-210 level, miR-39 was used for the spike-in. Renal miR-210 levels were normalized to RNU6, and circulating miR-210 levels were normalized to miR-39. Data are shown as mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001.