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Figure 3 | Molecular Medicine

Figure 3

From: IL-37 Suppresses MyD88-mediated Inflammatory Responses in Human Aortic Valve Interstitial Cells

Figure 3

MyD88 and TRIF have differential roles in mediating the inflammatory responses to TLR4 stimulation. AVICs were treated with MyD88 siRNA (100 nmol/L) or TRIF siRNA (100 nmol/L) for 48 h prior to LPS (0.2 µg/mL) stimulation. (A) Representative immunoblots show effective knockdown of MyD88 and TRIF. (B) Representative immunoblots and densitometric data show that knockdown of either MyD88 or TRIF reduced production of ICAM-1 following LPS stimulation, and knockdown of MyD88 had a bigger effect. (C) Cytokine data show that knockdown of MyD88 reduced production of all cytokines following LPS stimulation. Knockdown of TRIF markedly reduced IL-8 and moderately reduced MCP-1, but had no effect on IL-6. Data are mean ± SE. n = 6 experiments using cell isolates from distinct donor valves; *P <0.05 versus control; #P <0.05 versus LPS alone; ΔP <0.05 versus scrambled siRNA + LPS.

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