Figure 2

CCDC80 protein expression in human SGBS adipocytes during differentiation. (A) Western blot analysis of CCDC80 protein content in SGBS cell extracts of confluent preadipocytes (d 0), differentiating adipocytes (d 1–8) and mature adipocytes (d 14). (B) Confluent preadipocytes incubated with or without 25 nM dexamethasone, 0.5 mM IBMX and 2 µM rosiglitazone for 16 h. In A and B, a representative image is shown. Ratios of intensity of CCDC80 bands compared with intensity of GAPDH are expressed as a percentage of (untreated) confluent preadipocyte values and are means ± standard error of the mean from two experiments performed in triplicate. *P < 0.05 and **P < 0.01 vs untreated preadipocytes. (C) CCDC80 mRNA levels relative to 18S rRNA were measured in confluent preadipocytes, differentiating adipocytes (4 d post-differentiation) and mature adipocytes (14 d post-differentiation). Data are means ± standard error of the mean of 2^−ΔCp × 10³ from two experiments performed in triplicate. *P < 0.001 to preadipocytes; #P < 0.01 to differentiating adipocytes.