Figure 1

Characterization of Sirt6 protein expression in mouse tissues. (A) Western blot analysis of Sirt6 protein expression in various mouse tissues. Total proteins isolated from indicated mouse tissues (10 µg/lane) were subjected to electrophoresis and Western blot using rabbit antibody against mouse Sirt6, as described in Methods. Upper panel: autoradiograph of a representative immunoblot and total protein gel. Lower panel: densitometric analysis of the immunoblot data (normalized to total protein, n = 3). Results are expressed as mean ± standard error of the mean (SEM). (B) Cellular localization of Sirt6 in mouse intestines. Deparaffinized sections of the small and large intestines of normal mice were stained with immunofluorescence using rabbit antibody against murine Sirt6 or rabbit naïve IgG, as indicated. Nuclei were counterstained with DAPI. Sections were observed by immunofluorescence microscopy. SI, small intestine.