Figure 2

GTS21 treatment at reperfusion reduced infarct size and ROS and improved contractile function in vivo in a rat model of I/R. (A) Myocardial area at risk (AAR) as percent of LV area in rats receiving vehicle (veh, n = 6) or GTS21 (GTS, n = 6) (no statistical difference between groups); data are mean ± SE. Infarct size (INF) expressed as percent AAR. Statistical significance was determined by Student t test. *p < 0.01 versus I/R + veh. (B) Representative images of DHE staining of 8 µm frozen sections of LV free wall in sham, I/R + veh and I/R + GTS21 groups; 200 × magnification; excitation wavelength, 551 nm. (C) Quantitation of DHE fluorescence (AU, arbitrary units) in microscopic images using Image Studio Lite v3.1. Staining intensity was measured in three independent areas in three sequential sections per heart. n = 5 hearts/group; bar graph represents group mean ± SE. Statistical significance determined by one-way ANOVA. *p < 0.01 versus sham and I/R + GTS.