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Figure 3 | Molecular Medicine

Figure 3

From: Nicotinic Acetylcholine Receptor-Mediated Protection of the Rat Heart Exposed to Ischemia Reperfusion

Figure 3

Membrane potential of mitochondria isolated from perfused rat hearts. (A) MitoTracker red dye used to measure mitochondria membrane potential (ΔΨm) by flow cytometry. Scatter plot showing individual mitochondria sorted by side scatter (SSC) and fluorescence intensity of MitoTracker dye. Red dots are mitochondria from I/R hearts, blue dots are from perfusion control (PC) nonischemic hearts and green dots are from GTS21-treated I/R hearts. The established gating parameter is indicated by the black lines encircling the PC mitochondria. (B) Histograms represent numbers of mitochondria counted and the intensity of MitoTracker red staining of those mitochondria isolated from each treatment group: a, antimycin A-treated mitochondria (hypopolarization); b, perfusion control (PC); c, I/R + GTS21; d, I/R + veh; e, oligomycin A-treated mitochondria (hyperpolarization). (C) Bar graph quantifying the percentage of total mitochondria in each treatment group that fell within the gating parameters established for the perfusion control mitochondria. Values are mean ± SE; PC, n = 3; I/R + GTS, n = 6; I/R + veh, n = 6 hearts. *p < 0.01 versus all groups; significance determined by one-way ANOVA.

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