Figure 7

Effects of GTS21 on intracellular ATP. AC16 cells were exposed to 6-h hypoxia followed by 1-h reoxygenation (H/R)(+) or cultured under normoxia (-) conditions. Cellular ATP content was measured using a luciferase assay and normalized to total protein. (A) Cells were treated with GTS21 (1, 10 nM) only during reperfusion. (B) Pretreatment with MLA (100 nM) abolished the GTS21 effect on H/R cells. Data (mean ± SE) represents n = 3–6 cell culture dishes/treatment group. Each ATP assay data point was done in triplicate. Significance was determined by ANOVA post hoc Newman-Keuls; *p < 0.01 differences between groups as indicated by the connecting line.