CNI-1493 inhibits cytokine-inducible NO production in activated RAW cellsa
In this postactivation experiment, RAW 264.7 cells were stimulated for 8 hr with LPS and IFNγ as indicated, then the medium replaced, and CNI-1493 added to achieve the concentrations indicated for 4 hr. Nitrite production by these treated cells into fresh medium containing L-arginine (100 µM) and CNI-1493 was measured for the subsequent 12 hr period by assay for total nitrites accumulated in the culture medium. Data shown are nitrite production expressed in nmol/12 hr/106 cells (mean ± SEM, n = 3 experiments). Where the error bar is not visible, it lies within the extent of the symbol.