The dominant negative effect of mutant OK
Three natural TREs, (A) Lys, (B) ME, and (C) GH, were examined in HeLa and NIH3T3 cells at 10 nM T3. Cells were cotransfected with various WT:M ratios (i.e., 1:0, 1:1, 1:2, and 1:5) and treated with 10 nM T3 24 hr later. Experiments were performed at least twice, with duplicate plates within each experiment and results expressed as fold stimulation by 10 nM T3 over 0 nM T3 at each of the WT:M ratios ± SEM. The differences in fold stimulation between 1:0 and the other WT:M ratios were calculated for each cell type, and a significance value was obtained by Student’s paired t test. *The difference in dominant negative potencies between the cell types at the designated WT:M ratio is significant (p < 0.05).