Genomic Organization and Sequence of the Human NRAMP Gene: Identification and Mapping of a Promoter Region Polymorphism

Table 1 Intron (four flanking nucleotides)/exon (amino acids) boundaries and sizes (bp) for the 15 exons of human NRAMP identified by genomic sequence analysis of YAC-derived clones.

Exon Number	Size (bp)	Intron/Exon Boundaries			%AA Identity (Mouse)
EXON 1	155		Met Thr G		50
EXON 1	155		ATG .. 145bp .. ATG AC A G ly Asp Lys .. (43aa) … Lys Pro	gtga	68
EXON 2	143	acag	GT GAC AAG ………. AAA CCG Gly Thr .. (37aa) … Phe Lys	gtgg	95
EXON 3	123	acag	GGC ACC ………. TTC AAA Leu Leu .. (36aa) … Pro Lys	gtaa	100
EXON 4	120	acag	CTT CTC ………. CCt AAG Val Pro … (31aa) .. Ala Gly Ar	gtgg	91
EXON 5	107	tcag	GTG CCC ………. GCT GGA CG g Ile Pro .. (19aa) … Asn Tyr G	gtac	100
EXON 6	71	tcag	A ATC CCA ………. AAC TAC G ly Leu Arg .. (18aa) … Tyr Gin	gtgg	100
EXON7	68	gtag	GG CTG CGG ………. TAT GAG Tyr Val .. (48aa) … Val Lys	gtag	88
EXON 8	156	gcag	TAT GTG ………. GRC AAG Ser Arg .. (49aa) … Ala Ala	gtag	87
EXON 9	159	gtag	TCT CGA ………. GCT CGC Phe Asn .. (26aa) … Gln Gly	gtga	80
EXON 10	90	gcag	TTC AAC ………. CAG GGG Gly Val .. (36aa) … Met Glu	gtga	98
EXON 11	120	gcag	GGC GTG ………. ATG GAG Gly Phe .. (46aa) … Leu Leu	gtag	94
EXON 12	150	ccag	GGC TTC ………. CTG CTG Leu Pro .. (20aa) … Asn Gly Le	gtga	84
EXON 13	74	ccag	CTC CCG ………. AAT CCG CT u Leu Asn .. (47aa) … Tyr Leu	gtga	73
EXON 14	154	ccag	G CTG AAC ………. TAC CTG Val Trp .. (34aa) … Ter	gtac	67
EXON 15	108	ccag	GTC TGG ………. TAG

ISSN: 1528-3658