(a) Saturation binding plot of [3H]clozapine to rat brain membranes and (b) displacement curve and Scatchard analysis for unlabeled clozapine competing with [3H]clozapine.
Data in Panel a were plotted using Cricketgraph. Shown are total (—●—), nonspecific (—□—), and specific (—■—) binding in pmol/mg protein as well as Scatchard analysis of this data. In Panel b, data are presented as fraction of total specific binding defined by 1 µM unlabeled clozapine. The experiment was replicated three times.