Region | Kd (nM) | Bmax (pmol/mg) |
---|
Cerebral cortex | 6.7 | 0.24 |
Striatum | 7.3 | 0.17 |
Hippocampus | 4.3 | 0.11 |
- Rats were killed by decapitation, their brains rapidly removed and dissected on ice. Regions were homogenized separately and pellets were resuspended to approximately the same protein concentration (∼15 mg/ml). Binding was assessed at varying [3H]clozapine concentrations. Ki and Bmax values were determined by Scatchard analysis. Data are the means of three determinations with results varying less than 15%.