Activation of lyn kinase autophosphorylation by Taxol in C3H/OuJ (Lpsn) and C3H/HeJ (Lpsd) macrophages
Macrophages were treated as indicated in Materials and Methods with medium only or 100 ng/ml LPS. Results of the kinase assay were quantitated by Phosphorlmager analysis and relative autophosphorylation levels represent the geometric means of four identical experiments for C3H/OuJ macrophages (from Fig. 2) and five identical experiments for C3H/HeJ macrophages. Dashed horizontal line indicates the resting cell kinase expression level found in untreated cells. The standard error of the mean for Taxol-stimulated C3H/HeJ macrophages was ≤4% of the geometric mean for each timepoint.