Fig. 3

VHL expression in the developing murine central nervous system (E = 16.5 days) with light and corresponding darkfield images

(A and B) Prominent VHL expression in the neuroepithelium (ne) and nasal process (np) is evident with lower levels of VHL expression in the brachial arch (ba) (A, B: ×30). (C and D) Higher power views of the same structures revealing differential expression in the brachial arch (ba), neuroepithelium (ne), and nasal process (np) (C, D: ×60). (E–H) Low and high power views of the midbrain (mb), choroid plexus (cp), cerebellum (cb), pons (po), neuroepithelium (ne), and fourth ventricle (v) demonstrating elevated levels of VHL in the cerebellum and choriod plexus (cp) compared with other adjacent structures (E, F: ×60; G, H: ×100). (I and J) Views of the mid and anterior brain demonstrating the thalamus (th), midbrain (mb), and pituitary (pt), all with minimal levels of VHL expression (I, J: ×100). (K–O) Low and high power views of the developing eye revealing intense VHL expression in the retina (rt) and its outer neuroblastic layer (onl) in contrast to the lower levels of expression in the lens (le), vitreous humor (vh), and cornea (c). Panel O represents sense probe (K, L: ×125; M, N, O: ×250).