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Table 1 Oligonucleotides used for the amplification of human V H region genes a

From: Burkitt’s Lymphoma Is a Malignancy of Mature B Cells Expressing Somatically Mutated V Region Genes

Name

Sequence (5′ to 3′)

VH1L

TCACCATGGACTGGACCTGGAG

VH2L

ACCATGGACATACTTTGTTCCACGC

VH2L.2

ACCATGGACACACTTTGCTCCACGC

VH3L

ACCATGGAGTTTGGGCTGAGCTG

VH3L.2

ACCATGGAACTTGGGCTCCGCTG

VH4L

AAGAACATGAAACACCTGTGGTTCTTC

VH5L

ATCATGGGGTCAACCGCCATCCT

VH6L

ACAATGTCTGTCTCCTTCCTCATC

  1. aOligonucleotides VH2L and VH2L.2 as well as VH3L and VH3L.2 were used as a 1:1 mixture, respectively. The VH oligonucleotides hybridize to the leader region of the respective VH gene family.
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Molecular Medicine

ISSN: 1528-3658