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Fig. 6 | Molecular Medicine

Fig. 6

From: Identification of Galectin-3 As a High-Affinity Binding Protein for Advanced Glycation End Products (AGE): A New Member of the AGE-Receptor Complex

Fig. 6

Immuno- and ligand-affinity precipitation of surface radioiodinated RAW 264.7 cells

Detergent extracts prepared from surface radioiodinated RAW 264.7 cells were subjected to immunoprecipitation using isotype control rat Ig2a (Lane A), anti-galectin-3 mAb M3/38 (Lane B), or ligand-affinity precipitation using AGE-BSA-sepharose (Lane C). The precipitates were boiled in sample buffer containing 2.5% β-mercaptoethanol and electrophoresed through a 12% SDS-PAGE gel. Proteins were visualized by autoradiography.

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Molecular Medicine

ISSN: 1528-3658