Fig. 3

Effect of goat (A) or rabbit (B) PTHrP (1–34) antibody on LPS-induced lethality

(A) Male C57BL/6 5- to 6-week-old mice (n = 9/group) were injected i.p. with 200 µl goat PTHrP antibody (titer 1:8,000 by ELISA) (triangle) or goat control antibody (circle) 6 hr prior to LPS (700 µg i.p.). Statistical analysis of Kaplan Meier survival curves using either a log-rank (all time points equally weighted) or Peto & Peto Wilcoxon test (earlier time points more heavily weighted) showed that pretreatment with PTHrP antibody was protective (p < 0.030 and p < 0.031, respectively). (B) Mice (n = 8/group) were injected with 100 µl rabbit PTHrP antibody (titer 1:16,000) (triangle) or rabbit control antibody (circle) 6 hr prior to LPS (700 µg i.p.). Pretreatment with PTHrP antibody was protective (p < 0.004 log-rank; p < 0.003 Peto & Peto Wilcoxon).