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Fig. 5 | Molecular Medicine

Fig. 5

From: Vascular Gene Transfer of the Human Inducible Nitric Oxide Synthase: Characterization of Activity and Effects on Myointimal Hyperplasia

Fig. 5

Effect of iNOS gene transfer on ecNOS expression in SPAECs

(A) Northern blot analysis for ecNOS mRNA in total RNA samples from SPAEC, SPAEC-DFGiNOS grown in the presence of 0.5 mM L-NMA, SPAEC-DFGiNOS grown without iNOS inhibition for greater than 7 days, SPAEC exposed to 1 mM SNAP for 6 hr, and SPAEC exposed to 1 mM NAP for 6 hr. Human hepatocytes (HC) stimulated with a mixture of cytokines (30) serves as a negative control. (B) Western blot analysis for ecNOS protein expression in whole cell preparations of SPAEC, SPAEC-DFGiNOS cultured in L-NMA (SPAEC-DFGiNOS + NMA), and SPAEC-DFGiNOS permitted to synthesize NO for >7 days (SPAEC-DFGiNOS).

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