Fig. 5From: Vascular Gene Transfer of the Human Inducible Nitric Oxide Synthase: Characterization of Activity and Effects on Myointimal Hyperplasia Effect of iNOS gene transfer on ecNOS expression in SPAECs (A) Northern blot analysis for ecNOS mRNA in total RNA samples from SPAEC, SPAEC-DFGiNOS grown in the presence of 0.5 mM L-NMA, SPAEC-DFGiNOS grown without iNOS inhibition for greater than 7 days, SPAEC exposed to 1 mM SNAP for 6 hr, and SPAEC exposed to 1 mM NAP for 6 hr. Human hepatocytes (HC) stimulated with a mixture of cytokines (30) serves as a negative control. (B) Western blot analysis for ecNOS protein expression in whole cell preparations of SPAEC, SPAEC-DFGiNOS cultured in L-NMA (SPAEC-DFGiNOS + NMA), and SPAEC-DFGiNOS permitted to synthesize NO for >7 days (SPAEC-DFGiNOS).Back to article page