Expression of human iNOS in injured porcine femoral arteries in vitro
(A) Upper panel, X-gal staining for β-galactosidase activity in MFGlacZ-infected arterial segments. Blue arrows, positively staining (blue) cells located in superficial layers as viewed from the lumenal surface. Estimated infection efficiency about 1%. Lower panel, Immuno-localization for iNOS protein in DFGiNOS-infected arterial segments in cross section. Green arrows, positively staining cells which are isolated to the thin neointimal layer. No such staining is evident in the media. (B) Reverse transcriptase PCR amplification for iNOS and Neo expression in MFGlacZ- and DFGiNOS-infected balloon catheter-injured porcine femoral arterial segments 9 days after infection with retroviral vectors. The iNOS PCR product measures 316 bp, while the Neo product measures 728 bp. Reverse transcriptase PCR was also performed for β-actin expression as a control for the first strand cDNA synthesis reaction and PCR amplification (PCR product 652 bp).