Fig. 5
From: Nitric Oxide-Dependent Penile Erection in Mice Lacking Neuronal Nitric Oxide Synthase
Immunohistochemical localizations of eNOS in penes of wild-type and nNOS − mice
In cross sections of the proximal penis of the wild-type (A) and nNOS− mouse (B), staining is localized to the dorsal penile vasculature, peripheral regions of the corpora cavernosa, and the urothelium (arrowhead). Cavernous spaces (CS) are shown within the bilateral corpora cavernosa. Magnified views (C [from Panel A] and D [from Panel B]) show endothelial staining of the deep dorsal vein (V) and of dorsal arteries (A). A background level of staining is observed within dorsal penile nerve fibers (NF). In corresponding cross sections of peripheral regions of the distal penis, staining of the sinusoidal endothelium (arrows) is observed in the wild-type mouse penis (E) with a visibly increased staining intensity in the nNOS− mouse penis (F). In sections of the nNOS− mouse penis, Factor 8 immunohistochemical staining also localizes to sinusoidal endothelium (arrows; (G) while preabsorption with eNOS peptide blocks eNOS endothelial localizations (arrows; (H). eNOS immunoreactivity observed in urothelium (arrowheads; A, B, and E) is preabsorbed with eNOS peptide (H). Scale bars in Panels A and B = 300 µm; and in Panels C−H =100 µm.