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Table 1 Detection of HIV-1 DNA and RNA, and cellular RNA by in situ PCR in AIDS patients with no CNS symptomatology

From: AIDS Dementia Is Associated with Massive, Activated HIV-1 Infection and Concomitant Expression of Several Cytokines

 

HIV-1 DNA

HIV-1 RNA

iNOS RNA

TNF RNA

MIP-1α RNA

MIP-1β RNA

Case 1

      

Cerebrum

1+a

0

0

0

0

0

Case 2

      

Cerebrum

1+

0

0

0

0

0

Cerebellum

0

NDb

ND

ND

0

0

Case 3

      

Midbrain

0

0

0

ND

ND

ND

Cerebrum

1+

0

0

0

0

0

  1. aThe number of positive cells was determined by analyzing serial sections. If between 1 and 10 positive cells were identified in a 200 × field, the case was scored as 1+, between 11 and 25 positive cells, 2+, and greater than 25 positive cells, 3+. bND, not done.